Development and Application of Electrochemical Biosensors for Multianalyte Detection in Model Organ Systems

Abstract

The development and application of multianalyte electrochemical biosensors to detect metabolites is vital for understanding the cellular processes and mechanisms of toxicity in model organ systems. The need to accurately monitor health and diseases in these systems was the impetus for developing sensors that provided increased resolution, model organ system integration, customization, and multianalyte detection. Osmium polymer-based sensors showed increased resolution and were used to detect glucose metabolism with a background interferent, acetaminophen, in a model liver system, AML 12 cells. The eight-analyte amperometric biosensor provided customization and multianalyte detection to better understand mice macrophage metabolism in relation to endometriosis. Finally, a dual amperometric and potentiometric electrochemical biosensor was developed to provide customization and multianalyte detection in metabolism studies on mice peritoneal lavage samples after treatment that mimics an endometriosis phenotype. All of these systems allow for integration with model organ systems due to miniaturization and the use of a microfluidic pump and valve system and show the ability to analyze complex biological process.