Reflection and rotation for three dimensional microscopy of live cells

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dc.contributor.advisor Wikswo, John Peter
dc.contributor.author Wright, Charlie
dc.date.accessioned 2008-05-15T19:27:04Z
dc.date.available 2008-05-15T19:27:04Z
dc.date.issued 2008-04-18
dc.identifier.uri http://hdl.handle.net/1803/735
dc.description Highest Honors in Physics
dc.description.abstract Confocal scanning laser microscopy and multiphoton microscopy provide 3D data from biological specimens, but with limited z-axis precision. Multiple microscale mirrors can be used to obtain more accurate 3D data on living cells while using classical widefield microscopy. Etched silicon wells coated with aluminum were used to obtain 3D images of budding yeast cells, with information along the z-axis provided by reflections from the angled sides of the well. To supplement measurements obtained with this method, work was also conducted on a system to allow for rotation of a cell attached to a pulled glass tip. Images of a yeast cell obtained from either method can be fit to a simple 3D surface due to the cell's roughly spheroidal shape, which should provide accurate measurements of the volume of an individual budding yeast cell as it progresses through the cell cycle. en
dc.description.sponsorship William and Nancy McMinn Honor Scholarship, Vanderbilt Institute for Integrative Biosystems en
dc.format.extent 901601 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher Vanderbilt University. Dept. of Physics & Astronomy
dc.subject Reflection en
dc.subject 3d microscopy en
dc.subject MPWs en
dc.subject Rotation en
dc.subject.lcsh Yeast -- Analysis en
dc.subject.lcsh Biological specimens en
dc.subject.lcsh Cytology -- Technique en
dc.subject.lcsh Confocal microscopy en
dc.subject.lcsh Electron microscopy en
dc.subject.lcsh Three-dimensional imaging in biology en
dc.title Reflection and rotation for three dimensional microscopy of live cells en
dc.type Thesis en
dc.description.college College of Arts & Science
dc.description.department Department of Physics & Astronomy

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