Browsing by Author "Brandt Eichman"
Now showing items 1-20 of 23
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Brooks, Sonja Claudia (2014-06-25)Department: Chemical and Physical BiologyThe methylation state of DNA is important for gene expression, gene imprinting, X-chromosome inactivation, and transposon silencing in mammals and plants. DNA methylation is established by methyltransferases to mark a ...
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Karakaş, Mert (2011-12-09)Department: Chemical and Physical BiologyStructural information facilitates understanding of protein function and activity. The limitations of experimental methods for protein structure elucidation in applicability to certain types and families of proteins, ...
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Dimitrova, Yoana Nantcheva (2010-10-29)Department: BiochemistryThe mechanism controlling the switch between gene activation and repression is critically important for understanding the process of transcriptional regulation. Gene expression is highly controlled through a dynamic exchange ...
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Williams, Christina Kay (2012-03-28)Department: BiochemistrySimian Virus 40 uses its Large T antigen protein to bind and inactivate retinoblastoma tumor suppressor proteins, yielding cell transformation. T antigen is a modular protein with four domains connected by linkers. The ...
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Riddle, Abigail Leigh (2012-06-11)Department: Biological SciencesTelomeres are protective protein/DNA complexes that cap the ends of linear chromosomes. The repetitive, TG-rich telomeric sequences are elongated by the telomerase ribonucleoprotein. In Saccharomyces cerevisiae, the ...
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Stavros, Kallie Marie (2015-02-26)Department: Chemical and Physical Biology2-Amino-3-methylimidazo-[4,5-f]quinoline, IQ, has been identified as one of the most genotoxic materials according to Ames assays and animal studies. IQ is during high temperature cooking of meats and is classified as ...
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Qin, Ximing (2010-12-21)Department: Biological SciencesCircadian rhythms are intrinsic biological rhythms that have a period close to 24 hours. Prokaryotic cyanobacteria are the simplest organisms that show robust circadian rhythms, and are the only organisms that have a ...
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Pretto Garcia, Dalyir Imelda (2010-08-03)Department: BiochemistryReplication Protein A (RPA) is the primary eukaryotic ssDNA binding protein utilized in preventing secondary structure formation and re-annealing of unwound DNA strands, thereby controlling access to DNA templates in diverse ...
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Bass, Thomas Edwin (2018-11-21)Department: BiochemistryThe ATR kinase controls cell cycle transitions and the DNA damage response. Budding yeast contain three activators of Mec1ATR; however, only TOPBP1 is known to activate ATR in vertebrates. We identified ETAA1 as a replication ...
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Talley, Jennell Marie (2011-08-04)Department: Biological SciencesThe work presented in this dissertation focuses on a how the telomerase complex assembles both in vivo and in vitro and begins to explore how one member of the telomerase enzyme in the budding yeast, Saccharomyces cerevisiae, ...
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Robbins, Carol Bansbach (2012-06-15)Department: BiochemistryIn this dissertation I identify and define SWI/SNF, matrix-associated, actin-dependent regulator of chromatin, A-like 1 (SMARCAL1) as a genome maintenance protein. First, I introduce a functional genomic screen designed ...
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Pitts, Steven Lee (2011-07-29)Department: BiochemistryAll living organisms encode at least one type II topoisomerase. These enzymes help to regulate the superhelical density of the bacterial chromosome and remove knots and tangles from the double helix. Previous studies ...
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Brosey, Chris Arlen (2011-10-31)Department: BiochemistryThe integrity and propagation of the genome depends upon the fidelity of DNA processing events such as replication, damage recognition, and repair. Requisite to the numerous biochemical tasks required for DNA processing ...
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Badu-Nkansah, Akosua Agyeman (2016-07-21)Department: BiochemistryGenomic replication is a highly challenging task. The DNA replication machinery must precisely duplicate billions of base pairs while tolerating a multitude of obstacles including damaged DNA, collisions with transcriptional ...
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Leman, Julia Koehler (2012-07-23)Department: Chemical and Physical BiologyMembrane protein structures are very difficult to determine by solution NMR since severe line-broadening obstructs the measurement of restraints. To alleviate this problem we describe the measurement of paramagnetic ...
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Takar, Mehmet (2018-11-12)Department: Biological SciencesNeo1 was discovered in a multi-copy suppressor screen in Saccharomyces cerevisiae to search for factors conferring resistance against an aminoglycoside antibiotic, neomycin (1). Aminoglycoside antibiotics, used to treat ...
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Bhat, Kamakoti Prakash (2018-02-26)Department: BiochemistryThe replication stress response (RSR) maintains genome stability and promotes the accurate duplication of the genome. ssDNA binding proteins are integral components of the RSR and have been extensively studied for years. ...
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Adhikary, Suraj (2013-03-04)Department: Biological SciencesWork presented in this dissertation details structural and biochemical characterization of three yeast alkylpurine DNA glycosylases – Mag1 and Mag2 from S. pombe and Mag from S. cerevisiae. I determined high resolution ...
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Rubinson, Emily Holtzman (2011-02-17)Department: Chemical and Physical BiologyAlkylating agents covalently modify DNA bases to generate a chemically diverse set of lesions including cytotoxic N3-methyladenine (3mA) bases and mutagenic 1,N6-ethenoadenine (εA) and N7-methylguanine (7mG). To maintain ...
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Warren Jr., Eric Mason (2009-07-14)Department: Biological SciencesMcm10 is an essential eukaryotic DNA replication protein required for assembly and progression of the replication fork. Specifically, Mcm10 is required for the association of several replication proteins, including DNA ...