Browsing by Subject "DNA repair"

Now showing items 1-14 of 14

    • A Biochemical Characterization of the DNA Glycosylase DEMETER 
      Brooks, Sonja Claudia (2014-06-25)
      Department: Chemical and Physical Biology
      The methylation state of DNA is important for gene expression, gene imprinting, X-chromosome inactivation, and transposon silencing in mammals and plants. DNA methylation is established by methyltransferases to mark a ...
    • The Aspergillus fumigatus Mismatch Repair MSH2 Homolog Is Important for Virulence and Azole Resistance 
      dos Reis, Thaila Fernanda; Silva, Lilian Pereira; de Castro, Patricia Alves; do Carmo, Rafaela Andrade; Marini, Marjorie Mendes; da Silveira, Jose Franco; Ferreira, Beatriz Henriques; Rodrigues, Fernando; Lind, Abigail Lee; Rokas, Antonis; Goldman, Gustavo H. (mSphere, 2019-07)
      The genetic stability of every living organism depends on accurate DNA replication and repair systems. Here, we investigated the Aspergillus fumigatus MSH2 mismatch repair (MMR) gene MshA and how it impacts virulence and ...
    • Characterization of SRAP Domain DNA-Protein Crosslink Formation and Reversal with DNA Abasic Sites 
      Paulin, Katherine Amidon; 0000-0002-9311-9136 (2022-06-27)
      Department: Biochemistry
      Apuirinic/apyrimidinic (AP, or abasic) sites in DNA are one of the most common forms of DNA damage. AP sites are reactive and form crosslinks to both proteins and DNA, are prone to strand breakage, and inhibit DNA replication ...
    • DNA Damage Signaling Orchestrates SV40 Chromatin Replication 
      Sowd, Gregory Alan (2013-09-06)
      Department: Biological Sciences
      The effects of DNA damage signaling on the DNA replication of the polyomavirus SV40 are examined in this dissertation. Infection of monkey cells with SV40 results in large amounts of cellular DNA damage signaling through ...
    • DNA Lesions as Cellular Poisons of Topoisomerase II-alpha 
      Velez-Cruz, Renier (2005-11-02)
      Department: Biochemistry
      Topoisomerase II is an essential enzyme that controls DNA topology. This enzyme generates a protein-linked DNA break as a catalytic intermediate. This intermediate, known as the cleavage complex, is present in very low ...
    • Human DNA helicase B functions in DNA damage response and homologous recombination 
      Liu, Hanjian (2007-10-03)
      Department: Biological Sciences
      DNA damaging agents have been shown to stimulate the localization of human DNA helicase B (HDHB) in nuclear foci, suggesting that HDHB might participate in DNA damage response. In the first part of this dissertation, we ...
    • Human DNA helicase B in replication fork surveillance and replication stress recovery 
      Guler, Gulfem Dilek (2012-01-09)
      Department: Biological Sciences
      Correct and faithful genome duplication is crucial for preserving genomic integrity. Genome duplication is, therefore, highly regulated through a complex network of proteins that accomplish DNA replication in addition to ...
    • Regulation of replication fork stability by ssDNA binding proteins 
      Bhat, Kamakoti Prakash (2018-02-26)
      Department: Biochemistry
      The replication stress response (RSR) maintains genome stability and promotes the accurate duplication of the genome. ssDNA binding proteins are integral components of the RSR and have been extensively studied for years. ...
    • Replication Fork Remodeling by Helicase-Like Transcription Factor 
      Chavez, Diana Andrea (2018-06-26)
      Department: Biological Sciences
      During DNA replication, a moving replication fork can encounter various sources of replication stress that can lead the moving fork to stall. Organisms have developed various DNA damage response pathways that recruit and ...
    • S100P Is Selectively Upregulated In Tumor Cell Lines Challenged With DNA Cross-Linking Agents 
      jiang, fen (2005-09-09)
      Department: Pathology
      Bifunctional alkylating agents that cause DNA cross-linking are implicated in the pathogenesis of myelodysplastic syndrome (MDS) and MDS related acute myeloid leukemia (MDR-AML). Therefore, characterizing hematopoietic ...
    • SAN1 - a novel nuclease involved in interstrand cross-link repair 
      Andrews, Alex Michael (2018-07-11)
      Department: Cell and Developmental Biology
      The DNA damage response consists of complex signaling pathways that act to recognize and remove various types of genomic insults. One particularly dangerous type of lesion is an interstrand cross-link (ICL). ICLs are ...
    • Structural and biochemical characterization of yeast alkylpurine DNA glycosylases 
      Adhikary, Suraj (2013-03-04)
      Department: Biological Sciences
      Work presented in this dissertation details structural and biochemical characterization of three yeast alkylpurine DNA glycosylases – Mag1 and Mag2 from S. pombe and Mag from S. cerevisiae. I determined high resolution ...
    • Structural and Biochemical Studies of Alkylpurine DNA Glycosylase AlkD 
      Rubinson, Emily Holtzman (2011-02-17)
      Department: Chemical and Physical Biology
      Alkylating agents covalently modify DNA bases to generate a chemically diverse set of lesions including cytotoxic N3-methyladenine (3mA) bases and mutagenic 1,N6-ethenoadenine (εA) and N7-methylguanine (7mG). To maintain ...
    • Structural and Biophysical Characterization of the Nucleotide Excision Repair Factor XPA 
      Sugitani, Norie (2017-03-29)
      Department: Chemistry
      Maintaining the integrity of the genome is critical for the survival of all organisms. Genome maintenance must be efficient because we are constantly under exposure to genotoxic agents including UV-light, endogenous and ...