Browsing by Subject "DNA replication"
Now showing items 1-18 of 18
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(2021-02-12)Department: BiochemistryHuman cells are constantly exposed to endogenous and exogenous sources of DNA damage that cause thousands of DNA lesions in each cell every day. Accurate repair of these lesions ensures maintenance of genome stability and ...
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Analyzing genome integrity during DNA replication using iPOND (isolation of Proteins On Nascent DNA) (2013-07-12)Department: BiochemistryThe in vivo analysis of dynamic events that protect genomic and epigenomic integrity during DNA replication has been a challenging task. The proteins that ensure the accurate inheritance of the genome act directly at ...
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(2008-07-28)Department: BiochemistryThe replication of DNA occurs through a complex series of steps involving the coordinated action of many proteins. DNA polymerase alpha/primase (pol-prim) is a critical DNA replication factor that synthesizes short RNA-DNA ...
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(Frontiers in Microbiology, 2019-08-29)Bacteria, like all cells, must precisely duplicate their genomes before they divide. Regulation of this critical process focuses on forming a pre-replicative nucleoprotein complex, termed the orisome. Orisomes perform two ...
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(2012-06-11)Department: Biological SciencesTelomeres are protective protein/DNA complexes that cap the ends of linear chromosomes. The repetitive, TG-rich telomeric sequences are elongated by the telomerase ribonucleoprotein. In Saccharomyces cerevisiae, the ...
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(2019-02-12)Department: Chemical and Physical BiologyReplication of our genes requires unwinding of DNA and generating the new complementary strands for both leading and lagging parental strands. The DNA polymerases that generate the complementary strands require a short ...
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(2013-09-06)Department: Biological SciencesThe effects of DNA damage signaling on the DNA replication of the polyomavirus SV40 are examined in this dissertation. Infection of monkey cells with SV40 results in large amounts of cellular DNA damage signaling through ...
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(2013-04-09)Department: BiochemistryGiven the fundamental roles of histone deacetylases (HDACs) in the regulation of DNA repair, replication, transcription and chromatin structure, it is fitting that therapies targeting HDAC activities are now being explored ...
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(2006-10-06)Department: Molecular BiologyThis project was concerned with the functional components of mammalian DNA replication origins and how the misuse of a start site for DNA replication at the FMR1 locus might contribute to human Fragile X Syndrome. In ...
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(2012-01-09)Department: Biological SciencesCorrect and faithful genome duplication is crucial for preserving genomic integrity. Genome duplication is, therefore, highly regulated through a complex network of proteins that accomplish DNA replication in addition to ...
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(2006-04-12)Department: BiochemistryHUMAN TOPOISOMERASES AND DNA GEOMETRY: PUTTING A POSITIVE TWIST ON ENZYME ACTION AMY KATHLEEN MCCLENDON Topoisomerases play critical roles in maintaining DNA topology during cellular processes such as DNA replication ...
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(2005-04-19)Department: Biological SciencesBIOLOGICAL SCIENCES INSIGHTS INTO THE ROLES OF HUMAN CDC6 AND REPLICATION PROTEIN A IN INITIATION OF EUKARYOTIC DNA REPLICATION VITALY VLADIMIROVICH KLIMOVICH Dissertation under the direction of Professor Ellen H. ...
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(2019-05-15)Department: Biological SciencesDuring DNA replication the replication fork can become stalled by impediments that prevent complete and accurate duplication of the genome. DNA replication fork reversal is an important pathway for the protection and ...
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(2009-05-28)Department: BiochemistryGenetic instability is a hallmark of cancer. The ATR-ATRIP complex functions at the apex of a cell cycle checkpoint signaling pathway that is critical during every cell division cycle to maintain genome integrity. ATR ...
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(2018-02-26)Department: BiochemistryThe replication stress response (RSR) maintains genome stability and promotes the accurate duplication of the genome. ssDNA binding proteins are integral components of the RSR and have been extensively studied for years. ...
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(2014-04-17)Department: BiochemistryErrors during DNA replication lead to mutations which contribute to cancer development. To deal with these challenges, cells contain an innate machinery known as the replication stress response. ATR is the master regulator ...
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(2009-07-14)Department: Biological SciencesMcm10 is an essential eukaryotic DNA replication protein required for assembly and progression of the replication fork. Specifically, Mcm10 is required for the association of several replication proteins, including DNA ...
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(2013-11-19)Department: Biological SciencesFaithful DNA replication during each cell division cycle is carried out by multiprotein complexes acting at each replication fork. In this complex, DNA unwinding and synthesis activities, driven by the Mcm2-7 helicase and ...