| dc.creator | Yufenyuy, Ernest Limnyuy | |
| dc.date.accessioned | 2020-08-22T00:03:35Z | |
| dc.date.available | 2015-04-23 | |
| dc.date.issued | 2013-04-23 | |
| dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-03262013-155906 | |
| dc.identifier.uri | http://hdl.handle.net/1803/11446 | |
| dc.description.abstract | This dissertation discusses the role of intersubunit interfaces in HIV-1 capsid assembly and stability. In this study I showed the presence of both the NTD-CTD and CTD-CTD trimer interfaces in mature HIV-1 virions using engineered disulfide crosslinking. Using mutagenesis, I showed that these interfaces are critically important for assembly and stability of the HIV-1 capsid. I also showed that the CTD-CTD trimer interface is formed only after cleavage of CA-SP1. My results provide valuable fundamental knowledge about the biology of capsid assembly and stability and highlight the viral capsid as an attractive target for anti-HIV therapy. | |
| dc.format.mimetype | application/pdf | |
| dc.subject | Assembly | |
| dc.subject | HIV-1 | |
| dc.subject | Uncoating | |
| dc.subject | Capsid | |
| dc.title | The Role Of Intersubunit Interfaces In HIV-1 Capsid Assembly And Stability | |
| dc.type | dissertation | |
| dc.contributor.committeeMember | Mark Denison | |
| dc.contributor.committeeMember | Ellen Fanning | |
| dc.contributor.committeeMember | Ben Spiller | |
| dc.contributor.committeeMember | Chris Aiken | |
| dc.type.material | text | |
| thesis.degree.name | PHD | |
| thesis.degree.level | dissertation | |
| thesis.degree.discipline | Microbiology and Immunology | |
| thesis.degree.grantor | Vanderbilt University | |
| local.embargo.terms | 2015-04-23 | |
| local.embargo.lift | 2015-04-23 | |
| dc.contributor.committeeChair | Earl Ruley | |
