dc.creator | Miller, Sinead Emily | |
dc.date.accessioned | 2020-08-23T15:48:44Z | |
dc.date.available | 2015-11-23 | |
dc.date.issued | 2015-11-23 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-11202015-152348 | |
dc.identifier.uri | http://hdl.handle.net/1803/14665 | |
dc.description.abstract | Gold nanoparticles (AuNPs) were functionalized for rapid binding of Acinetobacter baumannii (A. baumannii), a Gram-negative bacterium. AuNPs were functionalized with colistin (Col), a polycationic antibiotic, using a two-step self-assembly process, in which heterobifunctional polyethylene glycol (PEG) was used as a linker. Colistin was successfully conjugated to the AuNPs (Col-PEG-AuNP), as validated by dynamic light scattering (DLS) and proton nuclear magnetic resonance (H1 NMR). Images taken using a scanning transmission electron microscope (STEM), in combination with x-ray energy dispersive spectroscopy (EDS), confirmed binding of Col-PEG-AuNPs to the cell wall of A. baumannii. Results generated from a rapid binding assay indicated that the reaction rate of Col-PEG-AuNP complexation with A. baumannii reached half-maximum saturation in approximately 7 minutes. Quantitative measurement of the kinetics of Col-PEG-AuNP binding to A. baumannii is essential to inform the design of colistin-functionalized magnetic nanoparticles for magnetic separation of nanoparticle-bound A. baumannii. | |
dc.format.mimetype | application/pdf | |
dc.subject | Lipopolysaccharide | |
dc.subject | Gram-negative | |
dc.subject | Multi-Drug Resistance | |
dc.subject | Gold | |
dc.title | Colistin-Functionalized Nanoparticles for the Rapid Capture of Acinetobacter baumannii | |
dc.type | thesis | |
dc.contributor.committeeMember | Frederick R. Haselton | |
dc.type.material | text | |
thesis.degree.name | MS | |
thesis.degree.level | thesis | |
thesis.degree.discipline | Biomedical Engineering | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2015-11-23 | |
local.embargo.lift | 2015-11-23 | |
dc.contributor.committeeChair | Todd D. Giorgio | |