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Reflection and rotation for three dimensional microscopy of live cells

dc.contributor.advisorWikswo, John Peter
dc.contributor.authorWright, Charlie
dc.descriptionHighest Honors in Physics
dc.description.abstractConfocal scanning laser microscopy and multiphoton microscopy provide 3D data from biological specimens, but with limited z-axis precision. Multiple microscale mirrors can be used to obtain more accurate 3D data on living cells while using classical widefield microscopy. Etched silicon wells coated with aluminum were used to obtain 3D images of budding yeast cells, with information along the z-axis provided by reflections from the angled sides of the well. To supplement measurements obtained with this method, work was also conducted on a system to allow for rotation of a cell attached to a pulled glass tip. Images of a yeast cell obtained from either method can be fit to a simple 3D surface due to the cell's roughly spheroidal shape, which should provide accurate measurements of the volume of an individual budding yeast cell as it progresses through the cell cycle.en
dc.description.sponsorshipWilliam and Nancy McMinn Honor Scholarship, Vanderbilt Institute for Integrative Biosystemsen
dc.format.extent901601 bytes
dc.publisherVanderbilt University. Dept. of Physics & Astronomy
dc.subject3d microscopyen
dc.subject.lcshYeast -- Analysisen
dc.subject.lcshBiological specimensen
dc.subject.lcshCytology -- Techniqueen
dc.subject.lcshConfocal microscopyen
dc.subject.lcshElectron microscopyen
dc.subject.lcshThree-dimensional imaging in biologyen
dc.titleReflection and rotation for three dimensional microscopy of live cellsen
dc.description.collegeCollege of Arts & Science
dc.description.departmentDepartment of Physics & Astronomy

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