dc.creator | Smith, Travis Kirk | |
dc.date.accessioned | 2020-08-22T00:21:59Z | |
dc.date.available | 2008-04-11 | |
dc.date.issued | 2007-04-11 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-04022007-172202 | |
dc.identifier.uri | http://hdl.handle.net/1803/11923 | |
dc.description.abstract | The work contained in this document describes the generation of new immunoreagents for Bves study, and our identification of a Bves-interacting protein. After generating new monoclonal antibodies to the Bves protein, I here describe the characterization of Bves expression throughout mouse embryogenesis, clearly demonstrating that Bves is widely expressed in many tissue types throughout gestation. I also describe the isolation of Geft as a directly interacting protein for Bves, and biochemically confirm and characterize the interaction between these two proteins. I then demonstrate a role for Bves in control of cellular motility via modulation of the Rac1/Cdc42 GTPases, likely through the Geft interaction. These findings represent both the first identification of a Bves-interacting protein and the first placement of Bves into a molecular pathway within the cell. The data presented here will undoubtedly serve as a departure point for many future investigations into the Bves protein, and represent seminal findings in the study of Bves function. | |
dc.format.mimetype | application/pdf | |
dc.subject | Cdc42 | |
dc.subject | Rac1 | |
dc.subject | Rho GTPases | |
dc.subject | antibodies | |
dc.subject | cell motility | |
dc.subject | Bves | |
dc.subject | GEFT | |
dc.title | Analysis of Bves function through identification of interacting proteins. | |
dc.type | dissertation | |
dc.contributor.committeeMember | David Bader | |
dc.contributor.committeeMember | Scott Baldwin | |
dc.contributor.committeeMember | Roland Stein | |
dc.contributor.committeeMember | Tsutomu Kume | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Cell and Developmental Biology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2008-04-11 | |
local.embargo.lift | 2008-04-11 | |
dc.contributor.committeeChair | Chris Wright | |